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Wonderful, Glen.
By: R. E. Tulloss (ret)
2017-07-09 22:42:10 PDT (-0700)

Thanks for sending the material this way, Glen.

By the way, we also got DNA from South African material labeled “pantherina.” However, it is not the European species. The DNA is most similar to an southern and eastern Asian species call Amanita subglobosa. We have very little information about it from the collector(s) and no photo. Do you see a pantherinoid species in your collecting area (possibly with European or Asian oaks)?

There is still more data to be processed. You may hear from me again.

Very best,

Rod

!!! :)
By: Glen van Niekerk (primordius)
2017-07-09 11:52:49 PDT (-0700)

Dear Rod,

This is great! I’ll forward you some information regarding possible contacts regarding a publication of the sequencing of Amanita foetidissima.

kind regards,

Glen

I have edited the previous comment to reduce claims concerning relationships.
By: R. E. Tulloss (ret)
2017-07-09 11:26:31 PDT (-0700)

We managed to get two fragments of nrLSU—about 610 characters attached to the nrITS sequence and another fragment of about 700 characters. These fragments also fall into the group of taxa cited in the previous comment. The nrLSU fragments seem less segregated from nrLSU sequences deposited in GenBank than was the case for the nrITS sequences.

Very best,

Rod

We have DNA!
By: R. E. Tulloss (ret)
2017-07-09 10:12:26 PDT (-0700)

This is the first time that I have seen DNA from what is very likely to be foetidissima. We have an nriTS sequence with a small number of indeterminate characters. Hopefully, I will have more to say later. The nrITS is similar to, but distinct from sequences derived from nauseosa (south eastern North America), manicata ss. Wolfe et al. (Hawaii), and nauseosa ss. Australian authors. Do you know of an publication from a South African researcher in which someone reported deriving sequences from foetidissima?

Thank you very much for sharing your material.

Very best,

Rod

Sadly, all the fruiting bodies are immature…not one single spore was seen.
By: R. E. Tulloss (ret)
2016-02-14 14:59:38 PST (-0800)

After an extensive search I found one basidium that was just starting to produce sterigmata.

The material dried well, and I have been able to examine the universal veil tissue extensively. The universal veil is extremely similar to the tissue I saw on the van Greuning (PRUM 2703) collection reported in the original description of foetidissima. There are clamps present in the universal veil tissue, the elongate inflated cells dominate the tissue and a very large percentage are under 115 um long.

The dried material stinks appropriately.

We will certainly try for DNA; however, if there is DNA in GenBank from this species, it is not deposited under the correct name. So we will not have helped with the goal of linking names to DNA sequences.

If we had spore data, we would be in good shape because we would have more confidence in the morphological determination of the species.

Thank you for all your efforts. They mean a great deal to me. I’m sorry that I cannot give you a more complete report.

Very best,

Rod

These certainly smell like something in the nauseosa group when dried.
By: R. E. Tulloss (ret)
2016-02-14 11:05:18 PST (-0800)

Very best,

Rod

Hello Glen
By: groundhog
2016-02-08 13:08:01 PST (-0800)

We have received your material and are in the process of accessioning it to Rod’s herbarium. Do you recall a distinct odor ??

Thanks,

Naomi

Hello, Glen.
By: R. E. Tulloss (ret)
2016-01-16 17:33:47 PST (-0800)

I would love to have some material. Thank you very much.

Very best,

Rod

By: Glen van Niekerk (primordius)
2016-01-16 13:04:03 PST (-0800)

Hi Rod,

I have not BUT I can send you some dried material as promised LONG ago. I have purchased a microscope (Second hand) but I am having problems with the condesor :(.. I have to get it fixed in the coming month.

Very interesting, Glen.
By: R. E. Tulloss (ret)
2016-01-16 09:31:07 PST (-0800)

Were you able to do any microscopy?

Very best,

Rod

Created: 2016-01-16 09:21:08 PST (-0800)
Last modified: 2018-01-06 09:46:37 PST (-0800)
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