When: 2017-02-09

Collection location: Parque de Monsanto, Lisboa, Portugal [Click for map]

Who: zaca

No specimen available

Notes:
I found this fungus for the 1st time almost three years ago. It was identified in the sequel of a topic posted at AscoFrance – http://www.ascofrance.fr/search_forum/28410 -
with the help of Alain Gardiennet and Björn Wergen. The latter also identified the specimens in observation 160806 by Elsa, also originated from Portugal, by the same time.
This fungus is quite variable in appearance and I didn’t recognize it until I saw a mature specimen in microscopy, but before I saw some immature ones. Referring to my first observation, the fruit bodies almost look as apothecia; most of the photos I have seen of this species, clearly are peritheciod; this time nothing that I could think as a fruit body. The specimens grew on the wood of a small shrub (Coronilla minima).
The distinguish feature of Nitschkia broomeana (Fracchiaea broomeiana is a synonym and considered as the current name in IF) is the hundred of spores in each ascus, with alantoid appearance and small dimensions (in my measurements all below 7 × 2 um).

Images

Microscopy-Part 1 (Immature specimen): Asci (x1000), in cresyl blue;
Photo that includes the specimens analyzed;
Microscopy-Part 1 (Immature specimen): Section (x100) and Hymenium (x400), in water;
Microscopy-Part 1 (Immature specimen): Hymenium (x400), in water;
Microscopy-Part 1 (Immature specimen): Hymenium (x400), in cresyl blue;
Microscopy-Part 1 (Immature specimen): Asci, bases of the asci and paraphyses tips (x1000), in cresyl blue;
Microscopy-Part 2 (mature specimen): Hymenium (x400), in water (top) and in chloral hidrate;
Microscopy-Part 2 (mature specimen): Spores (x400), and asci (x1000), in chloral hidrate;
Microscopy-Part 2 (mature specimen): Asci (x1000, in chloral hidrate;

Proposed Names

38% (2)
Used references: AscoFrance
Based on microscopic features
56% (1)
Recognized by sight: Asci look bitunicate. Not sure of what dothideomycete is polysporous with allantoid spores like this

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Comments

Add Comment
The asci look very strange for broomeana
By: Django Grootmyers (Heelsplitter)
2018-11-11 02:32:16 CST (-0500)

They appear to be bitunicate and tapered toward the apex. The asci in Fracchiaea broomeana appear to be very thin-walled and more clavate than this based on Nannfeldt’s pictures “here: http://www.ascofrance.fr/...;. The spores should also be arranged into a Chevron-like shape. You can see this in the pictures in my observation and Elsa’s.