Observation 271996: Graphis crebra Vainio

When: 2014-06-30

Collection location: Sintra, Portugal [Click for map]

Who: zaca

No specimen available

Notes:
(See Observation 271993)

SPECIES: Graphis crebra
DATE: 2014/06/30
SPECIMEN Nº: 6647
PLACE: Sta. Eufémia, Serra de Sintra, Sintra, Portugal
HABITAT: Mixed forest, dominated by Quercus trees
SUBSTRATE: Quercus tree
MICRO FEATURES

  • Striation: Entire
  • Carbonization: Lateral
  • Inspersion: Inspersed
  • Ascospores: Transverse – small
    • GROUP No.: 6

CHEMISTRY: K+y_r (yellow to red)
ASCOSPORES DIMENSIONS:
(19.4) 21.3 – 31.8 (32.9) x (5.4) 6.6 – 8.7 (9.4) µm
Q = (2.9) 3.1 – 3.76 (3.8) ; N = 32
Me = 26 × 7.4 µm ; Qe = 3.5

EVOLUTION IN THE KEY:
Group 6: Labia entire, excipulum laterally carbonized, hymenium inspersed, ascospores transversely septate:
1 Norstictic or stictic acid (K+yellow or K+yellow forming red crystals). . . . . . . 2
2 (1) Norstictic acid (K+ yellow forming red crystals). . . . . . . . . . . . . . . . . . . 4
4 (2) Ascospores small to very small(15–45 × 5–9 μm);lirellae variable. . . . . . . 5
5 (4) Disc exposed; lirellae erumpent, with lateral thalline margin, short to elongate and sparsely to irregularly branched (handelii- or scripta-morph). . . . . . . . . . . 6
6 (5) Disc white-pruinose (scripta-morph); pantropical . . . . . Graphis crebra Vain.
A. Ascospores 5–9-septate, 20–30 × 5–8 μm [holotype: Guadeloupe, Duss 541 (TUR-Vainio 27617!)] . . . . . . . . . . . . . . . . . . . . . . . . . . . . Graphis crebra Vain.
B. Ascospores 7–11-septate, 25–45 × 6–9 μm [holotype: Australia, Streimann 9367 (CANB!)] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Graphis apertella A. W. Archer

EXTERNAL LINKS:
http://www.tropicallichens.net/3397.html
http://www.discoverlife.org/mp/20p?see=I_RL112&res=640
http://www.seaveyfieldguides.com/...

Images

Microscopy: Apothecial sections;
Microscopy: Asci and spores.
Specimen-ink sketch

Proposed Names

87% (1)
Recognized by sight
Used references: Ref. 1 – Robert Lucking, Alan W. Archer, and André Aptroot : A world-wide key to the genus Graphis (Ostropales: Graphidaceae), The Lichenologist 41 (4): 363-452, 2009;
Ref. 2 – Robert Lucking: The taxonomy of the genus Graphis sensu Staiger (Ascomycota: Ostropales: Graphidaceae), The Lichenologist 41 (4): 319-362, 2009;
Ref. 3 – Robert Lücking, José L. Chaves, Harrie J. M. Sipman, Loengrin Umaña, and André Aptroot: A first assessment of the Ticolichen Biodiversity Inventory in Costa Rica: The genus Graphis, with notes in the genus Hemithecium (Ascomycota: Ostropales: Graphidaceae), Fieldiana Botany 46 (Jul 2008):1-126, 2009;
Ref. 4 – Zacarias Lepista and André Aptroot: Seven species of Graphis from Portugal reported new to Europe, The Lichenologist 48(4): 259–267 (2016).
Based on microscopic features
Based on chemical features

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