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31% (2)
Recognized by sight
71% (4)
Based on chemical features: The derived nrLSU sequence is a 100% match to GenBank’s L. pseudosensibilis KF030257 and to those of my own vouchers identified as such, obs 208874 and obs 215637

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= Observer’s choice
= Current consensus


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DNA sequencing results and discussion
By: I. G. Safonov (IGSafonov)
2018-02-02 20:23:59 CST (-0600)

> A clean and contiguous nrLSU sequence of 941 bps was obtained from this collection. The first base of this read corresponds to position 41 from the start of the LSU domain. There are no ambiguous characters present.

> A BLASTn search of this sequence gave the following list of top relevant hits (sorted by % identity):
KF030257: Lanmaoa pseudosensibilis voucher DS615-07 = 880/880 = 100% match
HQ161860: Boletus pallidoroseus voucher BD396 = 830/831 = 99.9% match (voucher likely misidentified)
KF030258: Boletus bicolor voucher snOH = 873/880 = 99.2% match (voucher likely misidentified)
KF030259: Lanmaoa carminipes voucher 4591 = 865/873 = 99.1% match

> The LSU of this collection is also a perfect match to the LSUs of my own vouchers of L. pseudosensibilisobs 208874 and obs 215637.

> The above BLASTn profile paints an interesting picture that I haven’t really elaborated upon before in connection with my sequenced collections of various Lanmaoa species:
While it appears that the identity of this collection (275044) as L. pseudosensibilis has been reasonably well established via matching its nrLSU sequence to those of the above 2 field-identified collections and also to a GenBank accession of presumably the same morpho-species, the identity of hits nos. 2 & 3 is not so clear cut. Though HQ161860 and KF030258 are likely to belong in Lanmaoa by virtue of their high identity scores, their associated vouchers have clearly been misidentified. Boletus pallidoroseus – also a member of Lanmaoa as per my own molecular research – has an LSU sequence that is only 97% to that of L. pseudosensibilis (e.g., obs 210760). One would also expect the LSU sequences of properly identified Baorangia bicolor to be less than 99% similar to those of species in Lanmaoa.
While challenges in field identification of Baorangia bicolor and various Lanmaoa species in North America have been plaguing GenBank to a large extent, causing all sorts of confusion and frustration, it appears that at least some taxa in Lanmaoa may naturally share very close LSU and/or TEF-1 sequences by virtue of perhaps being recently-diverged taxa. In other words, the barcode gaps of these loci are possibly lacking:
The above case is one such example.
The nrLSU of B. pallidoroseus/L. pallidorosea comb. prov. (MO210760) is a near match to that of L. carminipes (JQ327001). The TEF-1 of L. pallidorosea comb. prov. (MO283635) is a perfect match to L. pseudosensibilis DS615-07 (KF030407) – unless this is a labeling mistake in GB! – and a very close match to L. asiatica and L. carminipes MB06-001 (JQ327022).
The LSUs of B. carminiporus/L. carminipora comb. prov. (MO244944 & MO246870) are very close to those of L. angustispora (from Asia) and the North American L. roseocrispans (KP327615).
There are probably other examples for which no data exist in GenBank yet. Perhaps this phenomenon can be properly investigated for these and other loci in due course, provided that the genetic attributes of confidently identified vouchers continue to accumulate in the database.

Re change of consensus
By: I. G. Safonov (IGSafonov)
2018-02-02 17:48:25 CST (-0600)

Glad to see the vote of confidence. :-)
A detailed write-up will follow in the wings of this comment… I need to disclose some relevant details that I haven’t discussed before…

Created: 2017-04-27 22:23:05 CDT (-0500)
Last modified: 2019-10-15 21:54:29 CDT (-0500)
Viewed: 159 times, last viewed: 2019-11-09 09:20:48 CST (-0600)
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