Observation 278233: Panaeolus (Fr.) Quél.

When: 2017-06-05

Collection location: Wilkes-Barre, Pennsylvania, USA [Click for map]

Who: Dave W (Dave W)

No specimen available

Notes:
On a lawn where plenty of P. foenisecii grows. This solitary specimen has an interesting cap surface. Hoping to eventually see a thick spore print. Will scope the spores.

Images

One spore observed with width ~10 microns.

Proposed Names

58% (1)
Recognized by sight
-31% (2)
Recognized by sight: Gills are brown at maturity, stem is white, and spore print has a hint of purple-brown. If you still have the specimen, check the spores at 1000×.
29% (1)
Used references: Seems to match well with obs 136028.

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= Observer’s choice
= Current consensus

Comments

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I’m not certain about…
By: Dave W (Dave W)
2019-06-20 23:13:08 EDT (-0400)

the unit of measurement cited, but I believe I get the gist of it. Actually, gill material is not that difficult to work with. Although I gather that I probably sometimes mount too much material. I usually mount basidio tissue in Congo red, because it seems to accentuate details.

Use a small amount of material for the mount
By: Alan Rockefeller (Alan Rockefeller)
2019-06-20 13:33:51 EDT (-0400)

It is best to use just a couple micrograms of gill tissue for the mount – use an amount so small that if it was pure LSD crystal and you ate it, it would be a microdose. Mount it in KOH to help break up the cells, and press down firmly, but not hard enough to break the spores or cover slip with the end of a pencil or something. Using a really tiny bit of material will help the cover slip stay intact as you crush the material.

See observation 136028 to get an idea of what you will be seeing if it’s Panaeolus fimicola, especially the sulphidia.

If I find this…
By: Dave W (Dave W)
2019-06-20 09:07:10 EDT (-0400)

or something similar, I’ll look for sulphidia. My better scope (the binocular AmScope) should be sufficient to this task. Although, my ability to produce a decent section is a potential issue. Any suggestions?

Check for sulphidia on the gill faces
By: Alan Rockefeller (Alan Rockefeller)
2019-06-19 23:37:27 EDT (-0400)

That is a feature that P. fimicola has.

I don’t think this is P. foenisecii.
By: Dave W (Dave W)
2019-06-19 01:27:17 EDT (-0400)

1. For the print, I don’t see any brown/purple contrasting with the black strip. P. foenisecii prints all show distinct contrast, eg. obs 278232.
2. The spores in the micro are mounted in KOH. P. foenisecii spores mounted in KOH should have a brownish tinge.
3. Average spore quotient here appears to be in the neighborhood of 1.5. This is too small for P. foenisecii.

Champignons du Quebec…
By: Dave W (Dave W)
2017-06-06 21:52:50 EDT (-0400)

lists both cinctulus and subbalteatus as distinct species. (I had been under the impression they were synonyms.) Spore size/shape seems to be similar for these two species.

Created: 2017-06-06 00:58:17 EDT (-0400)
Last modified: 2019-06-20 23:13:09 EDT (-0400)
Viewed: 218 times, last viewed: 2019-08-22 23:08:47 EDT (-0400)
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