Observation 280056: Lanmaoa G. Wu et al.

Notes:
This species was the most abundant bolete at the foray in terms of the number of collections and the number of fruiting bodies found.
I was with a small group of people when when we found the fb directly to the right of the largest one in the thumbnail pic (it’s the same fb on bottom in pic #3). Later we found a collection of 3 fbs (the largest one and the two photographed on the boulder) growing in an area with exclusively deciduous trees; it took me a while to find the host tree, a red oak, as it was hiding behind the maples and black birches surrounding the collection.
The remaining 4 fbs – the two to the left of the largest one and the one one extreme right (all apparently single-fb collections as they were scattered on the ID table) – came from other foray participants and may not have originated from the same section of the woods.
My original diagnosis in the woods was Lanmaoa pseudosensibilis. I first began to suspect this was not pseudosensibilis when the exposed flesh blued vividly and uniformly and when the bluing faded away to light gray without changing to reddish-brown. Eventually I remembered to smell the mushrooms — all, with exception of the one with the base cut off (possibly a subvelutipes type even though it had yellow pores?), had an odor of curry. However, the chemical tests were inconsistent with either pseudosensibilis or sensibilis — KOH was dark mahogany brown to cherry red and ammonia was muddy yellow to greenish amber-yellow on the pileipellis and KOH was orangish-brown on flesh after the bluing faded.
Note the pores of the largest fb turning orange and the razor-thin tubes of the immature fbs. Dimensions of the largest fb:
> Cap = 7 cm across and 2 cm thick in the center
> Stipe = 6.8 cm long, 1.7 cm wide at apex and 3 cm wide at base
> Tube layer = 6 mm thick
Three collections were preserved separately. Two fbs were not saved — top left and last on right.

DNA Sequencing Results & Discussion:
> A clean and contiguous sequence of 610 bps was obtained from this material and uploaded to this obsie. There are no ambiguous characters.
> A BLAST search of this sequence gave a hit list featuring the top 13 entries (7 described & one undescribed taxa) from Lanmaoa. These are trusted vouchers, as most of them are featured in Wu et al. (2016).1 The highest achieved similarity is 97.6% with L. pseudosensibilis, a North American taxon which this collection resembles. The remaining hits have similarities in the 97.2—>94.7% range. Interestingly, a GenBank phylogram of the pertinent hits shows 280056 to be a “satellite” lineage to the core Lanmaoa, suggesting it might instead fall into a discrete but closely related clade.
> Currently there are 8 formally published Lanmaoa species in the world: borealis, carminipes, pseudosensibilis and roseocrispans from North America; angustispora, asiatica, and flavorubra from Asia; fragrans from Europe. All have been sequenced and accessioned into GenBank, with exception of fragrans.
Recently, I proposed the transfer of Boletus carminiporus and Boletus pallidoroseus into Lanmaoa based on the sequencing data of relevant vouchers:
1)obs 244944 & obs 246870 (as L. carminipora)
2) obs 210760, obs 247881, & obs 283635 (as L. pallidorosea).

1 https://www.researchgate.net/...

Species Lists

Images

Natural light, no flash
Natural light, no flash
Natural light, no flash
Flash deployed
Photographed immediately after sectioning; long exposure/no flash
Flash deployed; photographed within 1 min after sectioning
KOH reaction; flash deployed

Proposed Names

46% (2)
Recognized by sight
57% (1)
Based on chemical features: TEF-1-alpha sequence

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= Observer’s choice
= Current consensus

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Created: 2017-06-26 19:51:55 CDT (-0400)
Last modified: 2018-12-13 20:18:22 CST (-0500)
Viewed: 140 times, last viewed: 2019-07-13 08:26:26 CDT (-0400)
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