Observation 282780: Porotheleaceae Murrill

Growing on a well-rotted Fagus grandifolia log.

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Proposed Names

43% (5)
Recognized by sight
12% (2)
Recognized by sight
57% (1)
Recognized by sight

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= Observer’s choice
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multiple seq reads
By: Jonathan Frank (jonagus)
2018-05-24 11:43:38 CDT (-0400)

That would be a safe guess (or 1F-4B, etc). More important to have multiple seq reads to confirm: Or submit a separate piece of material to confirm. rather than a single read from a single PCR. knowing the PCR primers allows choices for multiple reads.

Not sure
By: Django Grootmyers (Heelsplitter)
2018-05-24 10:47:58 CDT (-0400)

I’m guessing this was probably done with ITS1F and ITS4 but I’m not sure. Stephen Russell sequenced this so he’d be the guy to ask.

re: ITS curiosity
By: Jonathan Frank (jonagus)
2018-05-23 18:44:51 CDT (-0400)

regarding the ITS query: this taxa could have a 100+ bp insertion in ITS1, but it would be good to know what PCR primers you used and if you obtained multiple sequence reads to confirm a contig, ideally from separate extraction and PCR. Alternatively it could be a PCR artifact, ie chimera.

Created: 2017-07-19 14:51:50 CDT (-0400)
Last modified: 2019-01-17 22:56:42 CST (-0500)
Viewed: 99 times, last viewed: 2020-07-02 04:01:12 CDT (-0400)
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