Notes:
This interesting bolete was found by Jim Barg (of NJMA) growing in a forest of black birch & beech. Hemlocks were across the stream at some distance away and thus are unlikely to be the host. The unusual habit and the unique set of morphological characters makes it tough for a generic placement. To me it gives an impression of a Retiboletus-Leccinum hybrid. This collection somewhat resembles an unusual bolete I encountered many years ago, obs 26487.

Notes by the collector (edited by me, where appropriate):
“Overall height about 4 inches, the caps were about 1-1.25” in diameter and maybe 3/8" thick at the apex. The stipes were about 3/8" in diameter and are VERY stiff and “woody”-feeling and did not break when bent lightly. The pore surface is white, non-staining. The stipe does not discolor on handling, and it seems more “deeply reticulate” than scabered. The cap is tomentose and lightly corrugated, and is non-viscid. I didn’t do any chemical tests on it, and these specimens were just too young and small to get a spore print. Overall, I’d call this one “very rugged” in terms of its makeup. The stipe is really stiff, much like Xerocomus tenax or Aureoboletus russelii."

DNA Sequencing Results & Discussion (posted 29-May-2018):
> A clean and contiguous 1451 bps nrLSU sequence was obtained from this collection. The first 18 bases of the LSU region are edited out. There are 4 ambiguous characters present: 2 x “R” (A/G) and 2 x “Y” (C/T).
> A BLASTn search of the first 963 bases (through to LR5 primer) gave a single very close hit (921/925 = 99.6% similar; the 4 mismatching bases are the ambiguities in MO285593) with #AY612811/Leccinum albellum voucher TH6968 collected in NC. The next 60+ hits (only 91-94% similar) are the accessions of various members of Leccinoideae other than Leccinum: Rossbeevera (53), Leccinellum(6), Turmalinea (2) and Chalmonixia (1). Unfortunately, a subsequent GenBank search for other accessions of Leccinum/Leccinellum albellum yielded no additional sequences.
> Based on the above BLASTn profile, this collection undoubtedly belongs in Leccinoideae, possibly in Leccinellum. This collection could be a morphological outlier of L. abellum after all. According to Bessettes’ BENA (p. 238), Prof. M.E. Smith of UFL has sequenced (ITS & LSU) many examples of phenotypically variable collections of L. albellum and confirmed them to be a single taxon despite suspicions to the contrary driven by the morphology. A request to compare this sequence with those in the possession of Prof. Smith has been recently communicated via Dr. Kudzma.

Species Lists

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Copyright © 2017 James M. Barg
Copyright © 2017 James M. Barg

Proposed Names

29% (1)
Recognized by sight
29% (1)
Based on chemical features: BLASTn search of the nLSU sequence
57% (1)
Based on chemical features: nrLSU sequence and BLASTn hits

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Created: 2017-08-08 17:58:00 CDT (-0500)
Last modified: 2019-06-18 23:43:59 CDT (-0500)
Viewed: 77 times, last viewed: 2019-07-12 02:10:19 CDT (-0500)
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