When: 2017-08-20

Collection location: Tsukuba, Ibaragi, Japan [Click for map]

Who: lxjfx

Specimen available

Notes:
Growing scattered under hardwoods (Quercus serrata) in the park

Cap felty, dark brown; pores reddish orange, becoming dark blue immediately when injured; stipe no reticulation; flesh light yellow, staining blue immediately when cut, becoming yellow with Ammonia; basal tomentum yellowish, brush-like

Species Lists

Images

IMG_20170820_103537.jpg
P8201627.JPG
P8201616.JPG
P8201615.JPG
P8201614.JPG
P8201613.JPG
P8201612.JPG
P8201610.JPG
Basal tomentum
IMG_20170820_104505.jpg
IMG_20170820_103545.jpg
IMG_20170820_103458.jpg
P8201624.JPG
Pileipellis
IMG_20170820_235422.jpg
Ammonia reaction on cap flesh

Proposed Names

1% (2)
Recognized by sight
56% (1)
Recognized by sight
Based on chemical features: TEF-1-alpha sequence and BLASTn results. Related to N. rubriporus and N. luridiformis.

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= Observer’s choice
= Current consensus

Comments

Add Comment
TEF-1-alpha sequencing results and discussion
By: I. G. Safonov (IGSafonov)
2018-01-21 18:39:34 PST (-0800)

> A clean and contiguous TEF-1-alpha sequence of 678 bps was obtained from this collection. The first 608 bases correspond to the EF1-983F —> EF1-1567R region (in the 5’ —> 3’ direction), including the primers themselves. There are no ambiguous characters; it’s a single haplotype.
> A BLASTn search of the above sequence gave a hit list consistent with this taxon being in the Neoboletus-Sutorius clade, a result that matches the macro-morphology-based placement. There are no close matches in GenBank, meaning that it’s not any of the taxa listed/described in the Wu et al. papers. 1,2 This might be an underscribed species, but I don’t really know much about even the published Boletaceae of Japan.
When the hit list is sorted by % identity and a tree is generated from the top 30 hits, 287326 nests between branches represented by accessions of Sutorius luridiformis (a European taxon) and Sutorius rubriporus (endemic to China).

References:
1 https://www.researchgate.net/...
2 https://www.researchgate.net/...

Created: 2017-08-19 22:48:12 PDT (-0700)
Last modified: 2018-12-14 09:48:07 PST (-0800)
Viewed: 184 times, last viewed: 2019-07-09 07:35:05 PDT (-0700)
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