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Done!
By: Alan Rockefeller (Alan Rockefeller)
2017-12-31 16:36:26 CST (-0600)
Thanks, Alan
By: I. G. Safonov (IGSafonov)
2017-12-31 10:09:13 CST (-0600)

Please do the same for obs 179586, obs 243052 and obs 250839.

Sorry about that!
By: Alan Rockefeller (Alan Rockefeller)
2017-12-31 09:30:23 CST (-0600)

Sequences have been destroyed.

Alan,
By: I. G. Safonov (IGSafonov)
2017-12-31 01:30:10 CST (-0600)

Did anyone ask you to add my sequences to this observation? I don’t think so.
I would appreciate if you refrain from doing something like this again.

Discussion of Sequencing Results
By: I. G. Safonov (IGSafonov)
2017-12-10 22:37:39 CST (-0600)

> A clean and contiguous nrLSU sequence of 909 bps was obtained from this collection and posted to this observation. The sequence begins from the 73 base from the the start of the LSU region. There is a single ambiguous character, a “Y” (C & T), so there are two haplotypes in this sequence.
A BLASTn search of this sequence gave a single, high-scoring and meaningful hit at 828/831 = 99.6% similarity with #HQ161857 Boletellus sp. from Costa Rica. The next several hits at 97% were vouchers of Puchroboletus species. Interestingly, Boletellus chrysenteroides (#KP327645) was only 96.2% similar.

> A clean and contiguous TEF-1-alpha sequence of 625 bps was obtained from this collection and posted to this observation. There are no ambiguous characters, it’s a single haplotype.
A BLASTn search of this sequence didn’t return any similar hits. The top-scoring hit at only ~93% was Boletellus indistinctus KT990736, a species from Asia. The next two hits at ~91% were also exotic Boletellus species from abroad.

> The are no sequences of B. pseudochrysenteroides in GenBank. Surprisingly, a brief analysis of BLASTn data revealed a dearth of high-scoring hits related to published Boletellus species that were part of recent phylogenetic studies by Halling et al. (2015) and Wu et al. (2014, 2016). However, B. pseudochrysenteroides is likely a member of Xerocomoideae, as evidenced by the hit patterns involving accessions from related genera (e.g., Puchroboletus and Aureoboletus) and probably clades in Boletellus, but more molecular data and a rigorous phylogenetic analysis would be necessary to establish its exact phylogenetic position in the Boletaceae. For now, it makes sense for pseudochrysenteroides to remain in Boletellus.

Salamat Igor!
By: Jon Shaffer (watchcat)
2017-09-09 17:34:45 CDT (-0500)
Spore ornamentation
By: I. G. Safonov (IGSafonov)
2017-09-09 17:06:38 CDT (-0500)

As expected, the spores are ribbed/longitudinally striate, just like in obs 288002. Good ID.

Created: 2017-08-25 22:33:54 CDT (-0500)
Last modified: 2018-12-13 19:28:35 CST (-0600)
Viewed: 209 times, last viewed: 2019-07-03 17:20:47 CDT (-0500)
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