Observation 288555: Lanmaoa G. Wu et al.

Notes:
A NJMA Foray collection. A single fruitbody/sole collection type brought to the ID tables by an unknown participant. Probably collected under oaks in a mixed forest of hemlock, white pine and various hardwoods.
The colors in the ‘in situ’ pix are accurate. A pronounced odor of curry was detected by several individuals and myself. All surface elements bruised/stained blue. The exposed context blued instantly throughout, very intensely in the bottom half of stipe and only weakly in the cap; the blue color completely faded away to brownish-gray within 15-20 min. KOH on cap = amber brown.
This species might be related to obs 280056 that was also found at Stokes S.F., at a location just two miles away, two months prior.

DNA Sequencing Results and Discussion:
> A clean and contiguous 610 bps TEF-1-alpha sequence was obtained from this collection and uploaded to this observation. There are no ambiguous characters, it’s a single haplotype.
> In light of the morphological similarity to obs 280056 mentioned above, the two TEF-1 sequences were aligned. Not surprisingly, they were found to be perfectly identical, meaning that the two collections are conspecific.
> For a discussion of pertinent BLASTn results, see obs 280056.
> A nrITS sequence was also obtained from this material. Unfortunately, the sequence became non-specific/heterogeneous in the middle (the end of ITS1, entire 5.8S and beginning of ITS2) for the total of 261 bases; however the first 172 bases and the last 257 bases were well resolved. Since all the haplotypes were of same length, the sequence could be read end to end in both directions.
A BLASTn search of both sequence fragments gave an almost perfect match to KY826075 associated with Boletales sp. voucher B2483 from Canada identified by Yves Lamoureux as Boletus sensibilis. Here is a link to the picture of that collection, courtesy of Renee Lebeuf: https://www.flickr.com/...

Species Lists

Images

Natural scattered light
Natural light + sunshine from right
Natural scattered light
Natural scattered light
Artificial light (lamp) + flash deployed
Artificial light (lamp) + flash deployed

Proposed Names

57% (1)
Recognized by sight
61% (2)
Based on chemical features: 1) TEF-1-alpha sequence & BLASTn search
2) Conspecific with obs 280056

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= Observer’s choice
= Current consensus

Comments

Add Comment
Dario,
By: I. G. Safonov (IGSafonov)
2017-12-01 22:48:56 CST (-0500)

This collection cannot be sclerotiorum because that taxon definitely doesn’t clade in Lanmaoa genetically. I’ve sequenced 3 loci of the real sclerotiorum — see obs 243879 for details. More to the point, the TEF-1 sequences of 243879 and 288555 are only 91% similar, meaning that they are not conspecific.
One for sure is that sclerotiorum and related taxa (e.g., obs 215764) will wind up in a brand new genus one day. However, like it is the case with Lanmaoa and many other monophyletic bolete clades, the disposition of this new genus within the current and incomplete phylogenetic framework of the Boletaceae is very much up in the air.