Species Lists

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Comparison Observation 288796
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Proposed Names

34% (3)
Recognized by sight
59% (2)
Recognized by sight: A variable-looking species with cap colors ranging from what’s seen here to almost white and equally mutable color of stipe ornamentation
Used references: my own obs 338899 & obs 210730
Based on chemical features: LSU matches several GenBank accessions, including that of obs 285593
1% (2)
Recognized by sight
Used references: C du Q

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= Observer’s choice
= Current consensus

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DNA results & discussion
By: I. G. Safonov (IGSafonov)
2019-06-02 17:29:52 PDT (-0700)

This collection gave a relatively weak DNA extraction and required repeated nested PCR. This has frustratingly been happening to a various extent – i.e., difficult PCR to outright failures to amplify the desired DNA – with many samples from the last shipment (not only for LSU but also for ITS), and there are no discernible patterns yet to figure out why this has been happening…
However, Dr. Kudzma was able to obtain a good LSU sequence out of this collection consisting of bases 41-981 of this locus (through to and including the LR5 primer). There are no ambiguous characters, it’s a single haplotype.
A BLASTn search of this sequence gave close matches to the following:
>AY612811 = Leccinum albellum voucher TH6968 = 923/925 = 99.78% similarity (C/T + A/G mismatches)
>MH620337 = Leccinellum albellum voucher FLAS-F-61741 (from NC) = 889/891 = 99.7% similar (C/T + A/G mismatches)
>MH620338 = Leccinellum albellum voucher FLAS-F-61742 (from NC) = 848/851 = 99.65% similarity (C/T + A/G mismatches + a gap in a poly-T motif)
>MH375873 = Leccinellum cf. albellum voucher Mushroom Observer #285593 (from NJ) = 937/941 = 99.57% similar (2 x “Y” + 2 “R” ambiguities in MH375873)

This collection…
By: I. G. Safonov (IGSafonov)
2019-03-01 17:37:58 PST (-0800)

…was also submitted for sequencing (LSU). This locus should be able to separate Leccinellum from Leccinum. LSU sequences of albellum are in GenBank, too.

Leccinum?
By: I. G. Safonov (IGSafonov)
2019-03-01 17:31:57 PST (-0800)

The corrugated cap, non-staining flesh and southern distribution favor albellum. I think VA (its western side, i.e. the Appalachian region) happens to be the southern geographical limit of distribution for true hardwood-associated scaber stalks, as the natural range of both birch and aspen extends into this region from the north. Even conifer-associated Leccinum (sect. Leccinum) don’t go much further south. I recall Geoff Balme lamenting about having never seen one in his bolete rich area (Raleigh, NC). Notably, Dario found these two true scaber stalks in the Charlttesville area: obs 290898 and obs 291161; the former has been submitted for sequencing.

Thanks, Igor
By: dario.z (dario13)
2017-08-31 19:59:13 PDT (-0700)

Mixed hardwoods forest.
Flesh: white not staining on exposure.

Nice, Dario
By: I. G. Safonov (IGSafonov)
2017-08-31 19:38:05 PDT (-0700)

A very cool and distinct-looking scaber stalk!
Details of the habitat? What color did the flesh stain on sectioning?

Created: 2017-08-31 19:29:21 PDT (-0700)
Last modified: 2019-07-05 13:17:47 PDT (-0700)
Viewed: 121 times, last viewed: 2019-07-05 14:18:18 PDT (-0700)
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