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Notes:
A single fruiting body growing in the sandy shoulder of Rt. 532 right at the NG entrance. The mushroom appeared a bit waterlogged, and that probably accounts for the weird color of the gills — ochraceous and then changing to pink post collection (see photos); however, the spore print is white. The turnip-shaped bulb bore a limbate volva (see pix), and the pale cream cut flesh did stain pale red at the juncture of the stipe/cap and along the edges of the stipe, but not in the cortex, from apex to the bulb. These properties point toward mutabilis as the only match, which has been found within 100 yards of this collection site in the past (e.g., obs 74393 and obs 289783).
The below dimensions have been recorded several hours post collection:
Cap = 5.3 cm wide and 0.7 cm thick in the middle.
Gills = 6-7 mm wide.
Stipe = 6.6 cm long, 1.3 cm wide at the apex, 2 cm wide just above the bulb, and 2.8 cm broad at the widest point of the bulb.
The overall height = 7.3 cm.
Preserved for RET.

Spore Data:
[20/1/1]; mounted in Melzer’s and measured in profile/side-view only (suprahilar appendage to the side).
L x W = (8.8-) 9.1~12.1 × 4.4~5.3 (-5.8) μm; L’ x W’ = 10.3 × 5.0 μm.
Q = 1.82~2.36 (-2.38); Q’ = 2.05.
Hyaline, amyloid in Melzer’s, thin-walled, equilateral and oblong in face-view, slightly inequilateral and oblong in side-view, elongate (9 spores) to cylindric (9 spores). I couldn’t find spores longer than 12.1 μm and wider than 5.8 μm. The Q values are upper-range elongate to mid-range cylindric and longer spores (>9.3 μm) are mostly cylindric; I could not find ellipsoid or low-range elongate spores. The size and shape of the spores are not consistent with mutabilis.

Species Lists

Images

Artificial light (lamp) + flash deployed
Artificial light (lamp), but no flash
Artificial light (lamp), but no flash
Artificial light (lamp) + flash deployed
Mounted in Melzer’s and viewed at x1000
Mounted in Melzer’s and viewed at x1000

Proposed Names

-57% (1)
Recognized by sight: Lepidella with a collared bulb (i.e., in subsect. Limbatulae), some reddish discoloration of the cut flesh
Based on microscopic features: Spore size and shape are not consistent with mutabilis
57% (1)
Recognized by sight: A lepidella with a limbate volva
29% (1)
Based on microscopic features: Matching dimensions & Q values

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= Observer’s choice
= Current consensus

Comments

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DNA sequencing results & discussion
By: I. G. Safonov (IGSafonov)
2021-01-18 22:58:35 CST (-0500)

A clean and contiguous nrLSU sequence fragment of 519 bps was obtained from this collection by Dr. Kudzma and uploaded by me to this observation. The sequence is derived from a single forward read (LSU0F primer). Another forward read originating from the downstream primer (LR3R) is short because it quickly becomes heteromorphic (triple peaks, no resolution of haplotypes possible). Furthermore, the two reads cannot be joined because the first read loses resolution well before reaching the LR3R primer area.
A BLASTn-100 of this sequence gives a 97.93% match to RET’s accession of Amanita praelongispora. Below this hit, % identity plummets to 93%. The LSU sequences of A. cylindrispora and A. mutabilis (RET data) are only 90% and 85.4% similar, respectively.
It’s possible that MO290020 represents a known (published) taxon from subsect. Limbatulae, e.g. A. limbatula or A. parva (based on the spore measurements), but unfortunately there are no genetic data available for comparison at this time.

Herbarium Rooseveltensis Amanitarum (RET) has received specimen.
By: mcmacher
2017-11-02 13:52:22 CDT (-0400)

We have received the dried specimen. Thank you. It is being accessioned in Herbarium Rooseveltensis Amanitarum as RET 802-1.

Great!
By: I. G. Safonov (IGSafonov)
2017-09-22 16:04:10 CDT (-0400)

Looking forward to seeing you there, Rod.

I hope to get out to a few NJMA forays after we are back from our travels.
By: R. E. Tulloss (ret)
2017-09-22 12:09:10 CDT (-0400)

Rod

Rod,
By: I. G. Safonov (IGSafonov)
2017-09-21 13:09:22 CDT (-0400)

Thanks for the info.
This and other collections I dried for you will be in your hands soon.
Hopefully one day we will find out what this critter’s DNA has to say.
It’s been both an interesting and unusual 2017 Amanita season in NJ, with many uncommon and cryptic species making repeated and welcome appearances. This year will go down in my memory as the year of atkinsoniana, canescens and submaculata. Plentiful rains in the second half of summer certainly made a difference in the Pine Barrens that had been baked by the sun early on… Wondering what the fall is going to bring. It would be nice to get some rain again for a change, as September has been really dry.

DNA might help. I don’t have my data accessible on this trip; however,
By: R. E. Tulloss (ret)
2017-09-21 11:41:08 CDT (-0400)

the website shows we have nrLSU from mutabilis (a pretty good sequence 1378 bp long). However, the website does not include data on why there is no nrITS sequnce for limbatula or mutabilis or why there is not nrLSU for limbatula. It seems to me (dangerous to try to dig for memory) that we did try to extract DNA from a speciment of limbatula collected by David W. There ought to be some notes on that on my home computer. We’re traveling with Mary’s computer.

Very best,

Rod

Rod,
By: I. G. Safonov (IGSafonov)
2017-09-19 12:21:37 CDT (-0400)

I checked out the descriptions of all sect. Limbatulae taxa from NA on your website and dismissed parva on account of it being exannulate. I guess one can apply the same logic to limbatula — though it has a PV, its structure is less robust than that of my mushroom.
Yes, I agree that the mushroom was young when collected; however, it blitzed through maturation, grew is size and dropped a decent spore print in the 8-10 hours following collection, though I wouldn’t call the spore print copious. I understand that the upper range of spore length could still have been affected by the borderline maturity of this fruitbody.
So what is this critter? Mutabilis made good sense from the standpoint of gestalt morphology (despite the weird color of the gills and the unusual shape of the bulb), the reddening context, and relative proximity to the collection site of bona fide mutabilis. However, the spore size and shape seem to eliminate this taxon from consideration… I wonder is DNA sequencing can shed light on the identity of this organism.

Did you consider A. parva?
By: R. E. Tulloss (ret)
2017-09-19 10:24:16 CDT (-0400)

R

Good job. It looks a little young. Maybe that affected spore size and shape…
By: R. E. Tulloss (ret)
2017-09-19 10:23:02 CDT (-0400)

and range of variation in those numbers.

The Limbatulae also include a few species with marginate bulbs.

Very best,

Rod

Rod,
By: I. G. Safonov (IGSafonov)
2017-09-18 16:54:00 CDT (-0400)

Your concerns regarding the morphology (gill color and shape of bulb) not being consistent with mutabilis are further supported by the spore data.

I’m a bit concerned about the pointed bulb and dark gills.
By: R. E. Tulloss (ret)
2017-09-18 10:29:26 CDT (-0400)

Very interesting.

With “standard hotel” internet access.

Rod

You have a good eye and good instincts, Igor.
By: Judi T. (AvidAmateur)
2017-09-16 19:24:55 CDT (-0400)

I’ll be surprised if the spores don’t confirm your ID:)

Thank you, Judy
By: I. G. Safonov (IGSafonov)
2017-09-16 19:17:21 CDT (-0400)

Actually, this specimen doesn’t do the species enough justice in terms of both size and beauty… I still need to measure spores dropped by this critter – I hope they fit the mutabilis range. :-)

That’s a hefty beauty and nice find, Igor.
By: Judi T. (AvidAmateur)
2017-09-16 17:00:32 CDT (-0400)

Also a super post, as usual. Very helpful info.

Thank you.
By: R. E. Tulloss (ret)
2017-09-16 16:12:08 CDT (-0400)

R