Notes:
Habitat/Location: Top of forest ridge, growing in thick mulch/leaves. In a dense mixed hardwood forest in Northwest Georgia.

Pores: Golden brown. Tube layer is quite deep (almost 2 cm towards center). The pores themselves don’t seem to bruise to blue, but the flesh bruises quickly.

Stipe: cream to white, slightly scaly. dark brown ring near top.

Cap: Large (around 10cm diameter). Light brown to gold with striking blue to violet bruising.

Spore print color: Hard to acquire, dark ash brown

Species Lists

Images

Proposed Names

28% (1)
Recognized by sight
62% (3)
Based on chemical features: The derived nrITS sequence is very close to that of the TYPE specimen

Please login to propose your own names and vote on existing names.

= Observer’s choice
= Current consensus

Comments

Add Comment
Lisa,
By: I. G. Safonov (IGSafonov)
2019-06-02 19:41:33 CDT (-0400)

My pleasure. Actually this one got sequenced earlier in 2019, not in 2017. My apologies for not getting this done sooner. This sequence will eventually be uploaded to GenBank as L. quercophilum and I will update the observation with a GB accession code.
By the way, I didn’t discuss this below, but there are a few Canadian accessions of this taxon in GenBank. They are all labeled as “Boletales sp.” followed by a collection code (e.g., “voucher RL022”). These sequences are somewhat shorter than ours and from the type collection, but overall they are a 99% match.
Very best — Igor

@Igor
By: Lisa Kimmerling (L_Kimmerling)
2019-06-02 16:33:11 CDT (-0400)

Thank you so much for the update and the thorough information! Also, thank you for getting this one sequenced back in 2017. I’m so excited to have found an outlier! I will be sure to update the ID on iNaturalist and JungleDragon.

Lisa

DNA results & discussion
By: I. G. Safonov (IGSafonov)
2019-06-01 18:04:12 CDT (-0400)

The full-length nrITS sequence of MO295969 is 785 bps long (ITS1 = 146 bps, 5.8S = 161 bps, and ITS2 = 478 bps). There is a single ambiguity, an “R” (A/G) at position #114. Following my proofreading request, Dr. Kudzma confirmed no changes to the original sequence.
As per the comment below, overall this sequence is a very good match to the ITS derived from the holotype of L. quercophilum (KC691207). The latter sequences has 6 ambiguities – all are an “N” (any base). The two sequences differ at 11 positions:
> KC691207 has two gaps in the mixed homopolymeric motif (bases #72-79) early in ITS1: CCC-A-TT vs. CCCCATTT in MO295969.
> N vs. T at #103
> N vs. R. at #114
> N vs. C at #258
> N vs. T at #319
> In the ITS2 region, bases #473-482 in MO295969 are AAATAAAATT, whereas KC691207 has a gap at #476, a “C” at #478, and an “N” at #482 (i.e., AAA-ACAATN).
7) C vs. T at #594
8) N vs. A at #638
Of these 11 mismatches only three are real/significant: #476, #478, and #594.

Hello, Lisa
By: I. G. Safonov (IGSafonov)
2019-06-01 16:06:18 CDT (-0400)

I finally got your bolete sequenced.
Dr. Kudzma obtained a nice full-length ITS sequence. It’s very similar (98.6% similar) to the ITS of Leccinellum quercophilum holotype. There are a number of differences, but more than half of them are ambiguous bases, not real mismatches, so the real similarity is over 99%. I asked Dr. Kudzma to proofread the sequence one more time to make sure there are no lingering errors.
There is nothing else so close in GenBank, so I am pretty certain your collection is L. quercophylum. Interestingly, this species thus far is known to be distributed in the northern tier of the country (east of the Mississippi River), so your collection is a notable geographical outlier.

Sample received
By: I. G. Safonov (IGSafonov)
2017-11-14 12:34:05 CST (-0500)

Thank you, Lisa, for sending in the material. It will eventually be submitted for sequencing.

Thank you!
By: I. G. Safonov (IGSafonov)
2017-10-28 11:05:44 CDT (-0400)

I am glad you saved it, L. Kimmerling, and thank you for posting, of course. There is nothing wrong for posting unknowns — folks come here for help all the time and they get it frequently as there are a number of excellent identifiers browsing the site.
I will send you my mailing address via MO right after I am done with this comment. If you don’t get it (some ISP’s block MO correspondence or mark it as SPAM mail), let me know through this observation.

Sample
By: Lisa Kimmerling (L_Kimmerling)
2017-10-28 10:39:42 CDT (-0400)

I try to be as descriptive as possible! I am still a newbie to mushrooming, but I am absolutely captivated by the fungal world! I apologize for posting so many Unknowns here on MO, but I’m still trying to wrap my head around all of the diversity out there!

I have a large chunk of the cap that I am drying out if you would like a sample. How would I go about sending it?

More
By: I. G. Safonov (IGSafonov)
2017-10-28 10:24:56 CDT (-0400)

If you save this collection, I would be interested in a sample for DNA sequencing.

You are welcome
By: I. G. Safonov (IGSafonov)
2017-10-28 10:23:09 CDT (-0400)

I am not quite sure what you have here as the fruitbody is quite old and beat up. By the way, thank you for a detailed description. The flesh of L. griseum, a European taxon, stains violet, but the same staining of the cap is rather unusual. Its inclusion in BENA is rather interesting. The taxonomy and nomenclature of this species “warrant review” as per Kuo et al. 1
1 http://www.mushroomexpert.com/..., pp. 328-329

Thanks!
By: Lisa Kimmerling (L_Kimmerling)
2017-10-28 07:42:34 CDT (-0400)

Thank you, @IGSafonov! I still need to acquire that bolete guide!

Created: 2017-10-27 11:14:25 CDT (-0400)
Last modified: 2019-07-05 16:16:31 CDT (-0400)
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