Notes:
I’m going to redo these for Danny as I had to leave for a bit and am not entirely happy with them, what beginner is? Hopefully this will give you a bit of an idea any other hints will be gratefully received – set in water.

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my mistake
By: Danny Newman (myxomop)
2017-11-29 12:43:21 CST (-0500)

here is the installation file:

https://drive.google.com/...

feel free to contact me privately using the following link:

http://mushroomobserver.org/observer/ask_user_question/181

I suggest we keep discussion relevant to this observation here, in the form of comments.

have a great trip. see you when you’re back!

Having problems with download,
By: Teresa (Teresa)
2017-11-29 04:20:42 CST (-0500)

heading off to the Snowy Mountains early tomorrow but taking the laptop so hope to get something worked out while away. Won’t be back until Monday and then only for 24 hrs. Is there a more private way to communicate?

Hope to get to the snow melt line, its a once a year thing for me, maybe find some interesting myxos there; naturally be looking for ascos also. Be in touch again asap.

By the way, a friend who lives further north found some ruby red Orbilia sp. I’ve never seen before. She is going to collect and send to me via another friend in January, can’t wait to get my fingers on them……LOL Hope you slept well……

and I’m soon to go to sleep
By: Danny Newman (myxomop)
2017-11-28 22:18:14 CST (-0500)

so see you tomorrow!

OK will have a go at this later today
By: Teresa (Teresa)
2017-11-28 20:36:37 CST (-0500)

have to head off for MRI so will let you know ASAP

Many thanks for taking me on Danny, I truly appreciate your assistance

ok
By: Danny Newman (myxomop)
2017-11-28 20:13:04 CST (-0500)

let’s try for both options then.

put your stage micrometer onto the stage, get the hashmarks in frame and in focus, and take a picture with your camera (I don’t know why I assumed you didn’t have one when there are micrographs right here in the dang observation!). download and install piximetre (http://ach.log.free.fr/Piximetre/Bin/Piximetre.zip). there is an english guide available at https://boletales.com/.... it is for an earlier version, but it will work just fine. once you have the program installed and running and you have taken a photo of the stage micrometer hashmarks at 100x (aka 1000x if you count the magnification factor of 10x eyepieces), you can calibrate piximetre to your exact setup and take measurements digitally.

let me know when you’ve gotten that far.

Camera on scope for micro shots, its a trinocular 5.0 MP
By: Teresa (Teresa)
2017-11-28 20:03:45 CST (-0500)

but I connect it to the TV via HDMI to view due to easier use.

Happy to send you specimens after we return from the le Tape in mid December but willing to try and learn everything else if you wish to teach long distance and be patient with me that is……

if you have a stage micrometer
By: Danny Newman (myxomop)
2017-11-28 19:55:09 CST (-0500)

you would normally be halfway there, but the lack of a reticle is a tough one. i’m assuming you don’t have a trinocular port and camera setup either. i don’t know how well something like piximetre works with cell phones, but we could try that, provided you own a camera phone.

or…… you could send me some of the specimen and I will work it up myself. that could save us a lot of hassle.

the white background is for a regular photograph. I use a spot plate to do my KOH extractions, but anything white and non-porous would be fine, like a ceramic plate.

welcome to the dark side! ascos are the best, no matter what anyone says.

You are a very positive worker and I thank you for that.
By: Teresa (Teresa)
2017-11-28 19:44:45 CST (-0500)

no my scope has not been calibrated, and no I do not have a reticle. I do have a stage micrometer but do not know how to use it yet, so I’m spending a lot of time searching the net trying to find answers as I have never had the luxury of a tutor or university training.

Currently I just use a slide with water or basic chemicles. How do I get a solid white background?

I must thank you for the studies you are doing. What I found online last night are indeed very interesting and Ascos have become my new favourites after 20 years of myxos…..

our first step
By: Danny Newman (myxomop)
2017-11-28 19:43:25 CST (-0500)

starts with Zhuang’s key to the possible genera:

what? no!
By: Danny Newman (myxomop)
2017-11-28 18:46:22 CST (-0500)

i’m actively working on this as we speak, reading papers and making comparisons. in the meantime, this is the best broad brush with which to paint this and many other observations.

i will say, however, that measurements of the microstructures you observed will probably be important. is your microscope calibrated and do you have a reticle?

also, could you post a photo of the KOH extractable pigment against a solid while background?

disappointing
By: Teresa (Teresa)
2017-11-28 18:32:26 CST (-0500)
Re done micros for Danny,
By: Teresa (Teresa)
2017-11-28 00:09:48 CST (-0500)

images show size of objectives eg 4x 10x etc eye piece used 15x finally found elliptic spores in last images just prior to oil being heated too much by lamp; all set in water can redo tomorrow night in other chemical if required. Just remember I’m a learner. Found your page on ASCO France which was most helpful to me thanks. Hope this helps you a little. T.

Created: 2017-11-27 22:00:06 CST (-0500)
Last modified: 2017-11-28 19:48:57 CST (-0500)
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