Notes:
In a park like setting under planted white pine and douglas fir. Pore surface bruised slightly red. Taste pleasant. No smell. Stem was not hollow. 3%KOH on pore surface flashed red, then brown after about 10 secs, then eventually black. Spore color in H2O and KOH was yellow brown.

Species Lists

Images

KOH about 10 seconds
Spores in water

Proposed Names

12% (3)
Recognized by sight
12% (3)
Used references: NA Boletes reports this species/variety for New York and New Jersey.
-8% (4)
Recognized by sight: Growing under eastern white pine

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= Observer’s choice
= Current consensus

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DNA results & discussion
By: I. G. Safonov (IGSafonov)
2019-03-22 18:19:11 PDT (-0700)

> A clean and contiguous nrDNA sequence of 707 bps was obtained from this collection by Dr. Kudzma.
The full-length nrITS sequence is 602 bps long (ITS1 = 206 bps, 5.8S = 161 bps, and ITS2 = 235 bps), is preceded by the last 19 bps of the nrSSU region and is followed by the first 86 bps of the nrLSU region. There are a total of 6 ambiguous bases in the ITS sequence: 4 x “Y” (C/T) and 2 x “R” (A/G). Two of these (both are a “Y”) are in the ITS1 region and the remaining 4 are in the ITS2 region. There could be as many as 64 haplotypic combinations (2 6). This sequence is posted to the observation.
> A BLASTn search of the full-length ITS sequence returned a hit list consisting of accessions of S. lakei in the first 20/21 top hits (there were many more below). The similarity range is 98.7-99.3% for these top hits. The lack of perfect matches can be explained by the ambiguities found in these sequences. A number of haplotypes are obviously shared.
> A BLASTn search of the full-length ITS sequence specifically targeting accession of S. spraguei revealed that the similarity between the two taxa is <90%.

My guess is…
By: Dave W (Dave W)
2019-03-19 10:59:12 PDT (-0700)

that lakei var. pseudopictus (which has been reported from eastern NA) will turn out to be the same a lakei. Regardless, this could turn out to be the first DNA-verified example of lakei in the est.

Nice!
By: billyd
2019-03-19 07:00:39 PDT (-0700)

Thanks for solving this mystery Safonov, I’m curious if that’s the first report for the east coast.

The ITS sequence…
By: I. G. Safonov (IGSafonov)
2019-03-18 21:30:25 PDT (-0700)

…derived from this material by Dr. Kudzma says it is Suillus lakei! Congrats to those who supported this proposal from the beginning. I changed my vote accordingly to reflect the verdict by the molecules.

Sample received!
By: I. G. Safonov (IGSafonov)
2017-12-21 14:20:06 PST (-0800)

A sample of the specimen received today — thank you.

Glad there is material to sequence
By: Terri Clements/Donna Fulton (pinonbistro)
2017-12-14 13:12:56 PST (-0800)

Host trees for both S. spraguei and S. lakei are documented with this collection so it seems plausible that it could be either taxon from that standpoint—especially as both trees were planted. From my experience collecting both in Arizona and Maine these look a lot more like S. lakei from a macro morphological standpoint. As compared to S. spraguei S. lakei has a more brown red rather than bright pink or rose red scaly cap that can become smooth in age, a thicker shorter and often clavate stem that is not as scaly as that of the former, and context that sometimes blues in the stem base. See Dave’s obs for great photos of S. spraguei: http://mushroomobserver.org/142973 and one of mine for representative photos of S. lakei: http://mushroomobserver.org/284396

Good
By: I. G. Safonov (IGSafonov)
2017-12-14 08:09:36 PST (-0800)

Thanks. I will send you my addy via MO momentarily. Let me know here if you don’t get it. Check spam mail, too, just in case.

I have a sample
By: billyd
2017-12-14 07:54:42 PST (-0800)

Its not much but I would be willing to send it to either of you if interested.

Dave,
By: I. G. Safonov (IGSafonov)
2017-12-13 17:13:43 PST (-0800)

Let’s wait for billyD to ascertain availability of the material.
Lakei var. pseudopictus hasn’t been sequenced — no accessions in GB. I think we can safely assume its ITS sequence should be identical or close to the parent taxon for the type also comes from western USA. Also, just in case, Django Grootmeyers is in possession of a var. pseudopictus voucher, obs 265052

I think there could be a reliable way of telling lakei and spraguei apart by measuring the spore width. The spores of the former rarely reach 4 µm, whereas in pictus one should find plenty of spores wider than 4 µm and up to 5 µm. BillyD will need to spend a bit of time with his advanced scope. :-)

I’m game, Igor.
By: Dave W (Dave W)
2017-12-13 16:05:12 PST (-0800)

But the observation says there’s no herbarium specimen.

Is it known whether S. lakei var. pseudopictus is a valid name having a discernibly different sequence from S. lakei?

Dave,
By: I. G. Safonov (IGSafonov)
2017-12-13 15:05:28 PST (-0800)

Thanks for the detailed info on the mycota found under the departed Douglas fur.

In light of the controversy surrounding the identify of this collection, the best way to resolve the conundrum is to have it sequenced. I think it would be fair to say that whoever is wrong about the ID should bear the costs. The ITS will the job just fine as, despite the morphological semblance between spraguei and lakei, the two have very different sequences.

Doesn’t look like spraguei to me.
By: Dave W (Dave W)
2017-12-13 14:19:59 PST (-0800)

Cap scales are more numerous on spraguei. Although, I’ve got to admit, old spraguei fruit bodies can be confusing.

Igor, I never found any mycorrhizal fungi under/near the old Doug-fir, only what I believe are saprobes: Chlorophyllum rhacodes, Rugosomyces carneus (formerly Calocybe carnea), Lepista tarda. Cyanoboletus pulverulentus used to occur between an apple and hickory tree, not far from the Doug fir, and this species has been on the decline in this spot since the Doug fir was cut down. But I doubt this species was growing in association with the Doug fir. Also, a species of Hortiboletus in the same area which has continued to thrive after the Doug fir was gone.

This looks more like S. lakei to me
By: Terri Clements/Donna Fulton (pinonbistro)
2017-12-13 11:11:46 PST (-0800)

Found this interesting bit on Wikipedia: “Both Douglas fir and Suillus lakei are non-native introduced species in Europe. The fungus has been found in several central and south European countries following the intentional introduction of Douglas fir.”
Makes sense.

Terri

Feeling kerfuffled
By: billyd
2017-12-13 10:33:35 PST (-0800)

Safonov you may be right, it was under white pine, but the mushrooms when picked were fresh and not that degraded from the elements, and the stipe doesnt match at all.

By: I. G. Safonov (IGSafonov)
2017-12-13 10:21:45 PST (-0800)

…is a hard pine associate. It’s very common in the NJ Pine Barrens where it found P. rigida very much to its liking. I don’t see S. decipiens outside of this habitat niche in my home state (NJ). I doubt it grows with Pseudotsuga, not to mention P. strobus.
I think this collection could very well be a late-blooming S. spraguei that has been battered by the weather/cold.

Good comments
By: billyd
2017-12-13 10:09:05 PST (-0800)

Terri the tree is 100% Douglas fir, they are commonly planted in PA as an ornamental. The photos may be deceiving, I do not recall any blue bruising or tinge, but the pores bruise red and flash red instantly with KOH. The cap and stipe match S. decipens very well, and I agree with Safonov its more likely to be a east cost species rather than S. lakei. I measured the spores during lunch today and although there is not much size variation between S. decipiens, sprageui, and lakei, according to Kuo lakei is the smallest and does not reach a breadth of 5um. These are easy 5um and some a bit more, so at the moment S. decipiens is a good bet. Additionally KOH on the cap does turn greenish black.

West Coast Suillus in the East
By: I. G. Safonov (IGSafonov)
2017-12-13 08:05:31 PST (-0800)

Some mushrooms are naturally holarctic. While it’s true that human activity has unintentionally spread certain mycorrhizal fungi well beyond their natural distribution range around the globe though purposeful introduction of their host trees (e.g., A. muscaria, A. phalloides, Suillus spp., and others) and both flourished in their new environs, it doesn’t necessarily mean that it will happen in all situations. I wonder, how many West Coast fungi, especially boletes, have been reported growing in the eastern part of the country on MO?
Dave, what kind of fungi did you find under your Pseudotsuga when it was in good shape?

Up until 2 years ago…
By: Dave W (Dave W)
2017-12-13 05:20:49 PST (-0800)

there was a mature Douglas fir in my back yard in PA. The tree has since been cut down, as it was dying.

Are you sure about the Douglas fir?
By: Terri Clements/Donna Fulton (pinonbistro)
2017-12-12 21:04:16 PST (-0800)

It’s native to western NA. Never heard of it being planted elsewhere or seen it east of the Rockies in any of our travels cross country. Also the bluing of the cap flesh in the last photo is not described for S. lakei. Sometimes there is a slight greening in the flesh of the stem base. I’ve never found S. decipiens but it occurs under pines east of the Rockies and has a partial veil and large angular pores but again its flesh doesn’t blue. I think the only Suillus with bluing flesh is S. tomentosus which does not have a partial veil.

When trees move so do fungi
By: billyd
2017-12-12 18:32:00 PST (-0800)

Good suggestion Dave, it does resemble Observation 265052 a bit, but the other observations seem different.

Created: 2017-12-08 13:42:06 PST (-0800)
Last modified: 2019-07-03 20:37:57 PDT (-0700)
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