Collection location: Salmon River State Forest, Colchester, Connecticut, USA [Click for map]
Growing under hemlock and birch.
Pileus: 8-15 cm wide, surface dry, velvety, gray to grayish-brown, margin incurved at first.
Pore surface: Pale yellow, becoming olive to dirty-yellow in age, staining blue at first and then fading to greenish blue.
Stipe: 5-12 cm long and 3-5 cm wide, somewhat equal or enlarged down to the base, sometimes with a pinched base, yellow near the apex, with some reticulation, red below down to the base with scurfy red-punctate areas.
Context: Whitish to pale yellow, bluing when cut
Mycelium color: White
Odor: Not distinctive
LSU BLASTn of MO319104
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|I’d Call It That||3.0||10.08||2||(the3foragers,IGSafonov)|
sum(score * weight) /
(total weight + 1)
A clean and contiguous nrLSU sequence consisting of the first 978 bases (through to and including the LR5 primer) was obtained from this material. There two ambiguous characters: a “Y” at position 203 and an “R” at position 207. Dr. Kudzma says he needs to confirm them with another read.
A BLASTn of the 856 bps fragment (bases 106—>961) gave the following two top hits:
AY612803 = Boletus inedulis voucher NCJ14; 853/856 = 99.6% match (the two ambiguous bases + 1 gap)
KF030301 = Boletus roseipes voucher 4515 (E.E. Both’s holotype?); 834/838 = 99.5% match (the two ambiguities, 1 mismatch & 1 gap)
The first hit is undoubtedly a misidentification and is obviously roseipes. This is because there are two reliable northeastern C. inedulis accessions that have been used in published literature, E.E. Both’s KF030281 and M. Binder’s JQ327013. They are 99.7% similar to each other and only ~97% similar to C. roseipes.
A phylogenetic tree consisting of 38 assorted accessions from the BLAST featuring some representative taxa from (top to bottom) Lanmaoa, Neoboletus, Rubroboletus and Caloboletus, with Pulchroboletus sclerotiorum as the outgroup, has been uploaded to this observation.
This is way too early for these mushrooms. I do not know what do we going to have when it it actually time for them to fruit.
Great pictures Robert.
This is an awesome collection!
Created: 2018-06-09 14:18:43 CEST (+0200)
Last modified: 2019-02-26 18:39:26 CET (+0100)
Viewed: 130 times, last viewed: 2019-07-21 01:57:06 CEST (+0200)