Observation 319104: Caloboletus roseipes

Notes:
Growing under hemlock and birch.
Pileus: 8-15 cm wide, surface dry, velvety, gray to grayish-brown, margin incurved at first.

Pore surface: Pale yellow, becoming olive to dirty-yellow in age, staining blue at first and then fading to greenish blue.

Stipe: 5-12 cm long and 3-5 cm wide, somewhat equal or enlarged down to the base, sometimes with a pinched base, yellow near the apex, with some reticulation, red below down to the base with scurfy red-punctate areas.

Context: Whitish to pale yellow, bluing when cut
Mycelium color: White
Odor: Not distinctive
Taste: Bitter

Species Lists

Images

These was taken about a week later of the same fungi.
These was taken about a week later of the same fungi.
These was taken about a week later of the same fungi.
Copyright © 2018 I. G. Safonov (IGSafonov)
LSU BLASTn of MO319104

Proposed Names

91% (2)
Recognized by sight

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= Observer’s choice
= Current consensus

Comments

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DNA results discussion
By: I. G. Safonov (IGSafonov)
2018-11-09 05:28:24 CET (+0100)

A clean and contiguous nrLSU sequence consisting of the first 978 bases (through to and including the LR5 primer) was obtained from this material. There two ambiguous characters: a “Y” at position 203 and an “R” at position 207. Dr. Kudzma says he needs to confirm them with another read.
A BLASTn of the 856 bps fragment (bases 106—>961) gave the following two top hits:
AY612803 = Boletus inedulis voucher NCJ14; 853/856 = 99.6% match (the two ambiguous bases + 1 gap)
KF030301 = Boletus roseipes voucher 4515 (E.E. Both’s holotype?); 834/838 = 99.5% match (the two ambiguities, 1 mismatch & 1 gap)
The first hit is undoubtedly a misidentification and is obviously roseipes. This is because there are two reliable northeastern C. inedulis accessions that have been used in published literature, E.E. Both’s KF030281 and M. Binder’s JQ327013. They are 99.7% similar to each other and only ~97% similar to C. roseipes.
A phylogenetic tree consisting of 38 assorted accessions from the BLAST featuring some representative taxa from (top to bottom) Lanmaoa, Neoboletus, Rubroboletus and Caloboletus, with Pulchroboletus sclerotiorum as the outgroup, has been uploaded to this observation.

Thanks
By: Robert(the 3 foragers) (the3foragers)
2018-06-11 15:08:22 CEST (+0200)

Thanks Guys.
This is way too early for these mushrooms. I do not know what do we going to have when it it actually time for them to fruit.

Wow!
By: dario.z (dario13)
2018-06-11 05:05:34 CEST (+0200)

Nice find,Robert

C. rosipes
By: Jason Bolin (j.bolin@outlook.com)
2018-06-09 22:28:15 CEST (+0200)

Great pictures Robert.

Very nice, Robert
By: I. G. Safonov (IGSafonov)
2018-06-09 16:56:48 CEST (+0200)

This is an awesome collection!

Created: 2018-06-09 14:18:43 CEST (+0200)
Last modified: 2019-02-26 18:39:26 CET (+0100)
Viewed: 130 times, last viewed: 2019-07-21 01:57:06 CEST (+0200)
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