Notes:
Collector’s_Name: Julie Jones

Species Lists

Images

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surrounding area

Proposed Names

78% (2)
Recognized by sight: probably Paxillus cuprinus
34% (2)
Used references: Genomic data

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= Observer’s choice
= Current consensus

Comments

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By: Julie Jones (foobear)
2019-04-12 01:10:25 UTC (+0000)

I’ll take your word for it, thank you

P. aff. involutus
By: Danny Miller (alpental)
2019-04-11 15:14:00 UTC (+0000)

Also, look carefully and you’ll see you matched 100% with something somebody called “Paxillus affinity involutus”, meaning even they knew it wasn’t P. involutus, but only something kind of like it. In the case of Paxillus, many of the things in Genbank labelled P. involutus are actually P. obscurosporus.

Don’t trust Genbank names
By: Danny Miller (alpental)
2019-04-11 15:09:40 UTC (+0000)

The names in Genbank are often wrong. They are simply what the person thought it was when they collected it, as a guide. For some difficult genera like Russula and Cortinarius, Genbank names are thought to be wrong something like 80% of the time or more! Blasting and comparing to Genbank names is unfortunately not the right way to try and ID a mushroom from DNA.

So how do you get an ID? Often you can’t, but in this case, there is a study by Jargeat et. al. on Paxillus that determined what the actual official Paxillus involutus is. They also described P. cuprinus and published the official DNA of that. This DNA is a pretty good match to the official P. cuprinus, and not a good match at all to the official P. involutus.

Blast report
By: Julie Jones (foobear)
2019-04-11 13:53:23 UTC (+0000)

Except the Blast report shows 100% match with involutus

https://mycomap.com/genetics/blast-search/a75t68k1014-r7951

1
Paxillus aff. involutus D. Deshazer DD535 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence
Species Name: Paxillus aff. involutus D. Deshazer DD535
1345.48 0 100 100% 0 JN661725

Paxillus cuprinus
By: Danny Miller (alpental)
2019-04-11 00:15:54 UTC (+0000)

The DNA is a pretty good match to a reference sequence of Paxillus cuprinus, and much further away from the others.