When: 2018-09-13

Collection location: Brooks Co., Georgia, USA [Click for map]

Who: VSUfungi

Specimen available
Collection number: VSUfungi GR12-3

Found on sandy soil
Cap is 5 cm broad
7 cm in length
White spore print
Slight sac like volva found beneath the soil

Species Lists


Proposed Names

28% (1)
Recognized by sight
82% (1)
Based on chemical features: 100% nrITS match to at least one collection of Amanita longicuneus.

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= Observer’s choice
= Current consensus


Add Comment
If the nrITS match is conclusive, then you have the first record of …
By: R. E. Tulloss (ret)
2019-03-22 16:39:05 CDT (-0400)

A. longicuneus from south of North Carolina and Tennessee.

Very best,


Your sequence is a 100% match for the nrITS of Amanita longicuneus (KY580766).
By: R. E. Tulloss (ret)
2019-03-22 16:30:48 CDT (-0400)

There is an error in your sequence, but it is outside of the nrITS locus.

Here is an annotation of your sequence:

nrSSU (3’ terminus) comprises characters: 1-27
ITS1: 28-206
5.8S: 207-367
ITS2: 368-528
nrLSU: begins at 529, but should start with “TTT” instead of “TT”

Since the nrLSU 5’ motif is rather well-conserved, I think that there was a failure to detect alll three “T’s” because of poor signal near the end of the reads. Could be wrong, but that’s my first guess.

Cool. Look to see if there is a substantial limubus internus inside the volva of your dried material.


Very best,


sequence added…
By: VSUfungi
2019-03-22 14:42:53 CDT (-0400)

…to observation.

Sequencing in progress
By: VSUfungi
2018-10-28 14:38:06 CDT (-0400)

Hi Rod,
This specimen was selected for sequencing by Mycoflora@VSU. We will link to the sequence as soon as it is in. Thanks for the checklist link!

This is a North American species and very probably not A. vaginata.
By: R. E. Tulloss (ret)
2018-10-19 15:40:50 CDT (-0400)

Is a part of this specimen available for DNA sequencing? I would very much like to know what it can tell us.

The number of probable species of Amanita sect. Vaginatae in the southeastern U.S. is growing very rapidly as people are beginning to collect the group and record data on MO and facebook, etc.

An illustrated checklist for Alabama, Georgia, and Florida is expanding here:


Entries in the list are linked to descriptions in the www.amanitaceae.org web site.

Very best,

Rod Tulloss