Notes:
Growing under pine with oak nearby.

Species Lists

Images

D01CC964-269C-4468-B0FE-64632948254F.jpeg
EB0B218B-A463-4DF9-9BF3-0702CFF0B46E.jpeg
26DAAB57-7468-4E29-B714-0BBA74C501F3.jpeg
6BF4A5C2-518B-44E4-B1DA-A0937A2E9126.jpeg
1BA73763-BC11-4DB3-B948-F7225DF926DB.jpeg
AC388714-E05D-41AA-A36D-1F8FDA924D69.jpeg
B0AC13E4-5826-4A92-A1D6-C5C86221C219.jpeg
The youngsters have bright yellow flesh that quickly sirens greenish blue and then dark gray-blue

Proposed Names

28% (1)
Recognized by sight
45% (2)
Recognized by sight
Based on chemical features: Conspecific with obs 254746 by LSU

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= Observer’s choice
= Current consensus

Comments

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DNA results & discussion [edited]
By: I. G. Safonov (IGSafonov)
2020-12-01 13:06:17 CST (-0600)

A clean and contiguous nrDNA sequence of 1572 bps was obtained by Dr. Kudzma from this collection. There are no ambiguities, it’s a single haplotype.
The forward read (LR0R primer) gave the greater bulk of the LSU fragment, whereas the reverse read (LR22 primer, which is downstream of LR0R in the forward direction) gave the first 319 bps of nrLSU and the last 594 bps of nrITS (i.e., partial ITS1, and full-length 5.8S and ITS2 regions). Hence, the two reads overlap in LSU (for 247 bps). The LSU sequence consists of the first 978 bps, i.e., from the start of the locus through to and including the LR5 primer area. Following Rod Tulloss’ example, I posted the data as a single ITS-LSU sequence here, and that’s probably the way I intend to submit it to GenBank.
While the ITS sequence gave no matching or close hits in GenBank (the top hit at 95.3% identity and 81% query cover is Renee Lebeuf’s ‘Boletales sp. voucher RL005’, #KY826161, from Canada), the LSU sequence is a 100% match to that of Geoff Balme’s obs 254746 (https://www.ncbi.nlm.nih.gov/nucleotide/MH236164).

DNA results
By: I. G. Safonov (IGSafonov)
2020-11-27 17:22:43 CST (-0600)

I have a nice, clean LSU sequence derived from this collection by Dr. Kudzma (to be posted here at a later time). It’s a 100% match to the sole exemplar of the taxon that has been sequenced in the past — Geoff Balme’s obs 254746 from NC! I don’t think we have this particular species in the northeast (though N. brunneosquamulosus nom. prov. occurs in NJ/PA and LA) and the two data points we have now probably establish its north/south range pretty accurately. Still, it could be present in VA, and perhaps Dario Z. has already found it for me, but then Virginia represents the southern range of some of our known northeastern Neoboleti, such as the “chameleon” (the sister taxon of N. subvelutipes) and B. vermiculosoides.

Coming your way soon.
By: Logan Wiedenfeld (LoganW)
2018-10-24 22:23:40 CDT (-0500)
Thanks for posting, Logan
By: I. G. Safonov (IGSafonov)
2018-10-24 22:21:33 CDT (-0500)

Yes, it looks new to me, too, if I understand anything about southern/Gulf coast Neoboletus just by looking at the pix. I think this is probably the best-looking one of the LA bunch… I presume it’s coming my way, too. :-)

Haha
By: Logan Wiedenfeld (LoganW)
2018-10-24 22:10:02 CDT (-0500)

I think this is indeed a new one. I’ve seen it once before but was so ashamed of my abysmal photography that I didn’t post an obsie.

This place has all sorts of weird boletes. I encounter something new nearly every time I visit.

Another Neoboletus?!
By: I. G. Safonov (IGSafonov)
2018-10-24 21:58:18 CDT (-0500)

No end in sight for these types and not enough money in the world to sequence them all. :-)