Observation 34367: Hohenbuehelia grisea (Peck) Singer

When: 2010-02-12

Collection location: Los Trancos Preserve, Palo Alto, California, USA [Click for map]

Who: Douglas Smith (douglas)

Specimen available

Found on the bark of live oak.

The micro-shot is of the gill edge at 400x in KOH. The metuloid cystidia here are numerous. The cystidia are kinda interestingly encrusted, many encrusted in the top half, ending in a hard line.

Adding more micro details:

The second micro-shot is of the squashed gill edge at 1000x in congo red. Here are the smaller cystidia with a single projection.

The third micro-shot is of the squashed gill face at 1000x in congo red. Here are the encrusted metuloid cystidia.

The forth micro-shot is of spores from the gill at 1000x in congo red. The ave. spore size is : length – 7.16 +/- 0.43 (err 0.17) um, width – 5.77 +/- 0.58 um (err: 0.22) – q : 1.25 +/- 0.11, on 8 spores.

With the single projections to the smaller cystidia, from Funga Nordica this matches – H. grisea. It seems some sources have Hohenbuehelia atrocaerulea var. grisea and other have Hohenbuehelia grisea. And Index Fungorum call these synonyms. So, anyway this seems to be a good id, even though others seem to find this id fairly questionable or uncertain.



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Add Comment
nice Doug.
By: Debbie Viess (amanitarita)
2010-03-17 07:50:58 PDT (-0700)

OK, I am feeling the pressure to return to my scope…;)

Ironically, IMO, those encrusted cystidia actually showed up better in the unstained version!

By: else
2010-03-10 11:29:53 PST (-0800)

Hohenbuehelias have two types of cheilocystidia – those big thick-walled ones with the crystals which take front page, but also, small one with 2 or 3 finger like appendages. you have to squash the slide a bit to get to see those properly

no worries Doug, we can share the pain…
By: Debbie Viess (amanitarita)
2010-03-10 08:44:45 PST (-0800)

I’ll use congo red on my examples of this one from San Diego.

Didn’t use Congo Red
By: Douglas Smith (douglas)
2010-03-10 08:15:36 PST (-0800)

Well, there are a number of reasons. First perhaps, is that I wanted to see that the spores were hyaline, so I could tell that these weren’t Crepidotus with some tan or pigmented spores, and congo red could obscure that. Also I had been looking at Psathyrella and Inocybe lately (boy the life I lead!), and I wanted to be able to see if the cystidia walls were pigmented. And in the end, because I just hate to use congo red, I just do…

Now that I know that there are no pigmentation to look for at all, which I didn’t before hand, using congo red seems obvious. And I should try to use it in the future, if I get back to looking at these… which will probably never happen. And I’ll still hate to use congo red…

Cool stuff
By: Dimitar Bojantchev (dimitar)
2010-03-10 07:43:18 PST (-0800)

Very cool stuff. Why not use a drop of a colorizer on the micro shot? Congo Red works great in such cases..


Created: 2010-02-28 06:12:02 PST (-0800)
Last modified: 2018-11-08 23:05:54 PST (-0800)
Viewed: 401 times, last viewed: 2018-11-08 23:14:12 PST (-0800)
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