When: 2016-08-12

Collection location: Pine River Campground, Bayfield, Colorado, USA [Click for map]

Who: Django Grootmyers (Heelsplitter)

Specimen available

Notes:
Growing from a cut trunk of a large, living Pseudotsuga menziesii var. glauca. Annulus absent. Pileipellis a hymeniderm with hyaline, rounded cellular elements. Measurements of pileipellis elements: (15.3) 16.3 – 31.3 (37.4) × (12.1) 15.2 – 21.6 (24.9) µm, Q = 1 – 1.5 (2); N = 30, Me = 24 × 18.8 µm; Qe = 1.3. At least some clavate, thin-walled caulocystidia present. One caulocystidium measured at 49.59 × 7.80 µm. Apically thick-walled cheilocystidia and pleurocystidia abundant. Cheilocystidia and pleurocystidia did not seem to differ in any significant way. Cystidia not encrusted and without globules. Pleurocystidia measurements: (43.2) 45.3 – 59.5 (61.8) × (10.6) 14 – 17.6 (17.7) µm, Q = 2.6 – 4.2 (4.5); N = 10, Me = 50.3 × 15.7 µm; Qe = 3.3. Pleurocystidia walls in apically thickened portion 1.07 – 5.35 µm wide (30 measured. Basidia clavate, 4-sterigmate and basally clamped. Basidia measurements: (25) 26.3 – 34.9 (37.4) × (6.4) 7 – 8.6 (8.7) µm, Q = (3.1) 3.6 – 4.6 (4.7); N = 20, Me = 31.5 × 7.8 µm; Qe = 4.1. Spores smooth and almost hyaline when mounted in NH4OH (50%), phloxine (25%) and congo red (25%). Many spores with one large guttule. Spore measurements: (7.4) 7.7 – 8.6 (8.8) × (3.9) 4.5 – 5.1 (5.2) µm, Q = 1.6 – 1.8 (2); N = 30, Me = 8.2 × 4.8 µm; Qe = 1.7

Species Lists

Images

WP_20160812_12_57_37_Pro__highres crop.jpg
WP_20160812_12_57_23_Pro__highres crop.jpg
WP_20160812_12_55_09_Pro__highres.jpg
DSC_9690 Gill edge at 400x in NH4OH (2 drops), congo red (1 red) and phloxine (1 drop).JPG
DSC_9697 Pleurocystidia, basidia and spores at 400x in NH4OH (2 drops), congo red (1 red) and phloxine (1 drop).JPG
DSC_9707 Basidia and spores at 400x in NH4OH (2 drops), congo red (1 red) and phloxine (1 drop).JPG
DSC_9727 Lamellar trama, basidia, pleurocystidia and spores at 400x in NH4OH (2 drops), congo red (1 red) and phloxin...
DSC_9734 Pileipellis at 400x in Melzer's.JPG
DSC_9871 Stipe surface with caulocystidia at 100x in NH4OH (2 drops), congo red (1 red) and phloxine (1 drop).JPG

Proposed Names

57% (1)
Used references: Largent, D. L., & Baroni, T. J. (1988). How to Identify Mushrooms to Genus VI: The Modern Genera (Vol. 6). Mad River Press Inc.
Smith, A. H. (1972). The North American species of Psathyrella. Memoirs of the New York Botanical Garden 24: 1-633.
Vašutová, Martina. (2008). Taxonomic studies on Psathyrella sect. Spadiceae. Czech Mycology, 60(2), 137-171.
Based on microscopic features

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