When: 2019-10-26

Collection location: Forest Resource Education Center, Jackson Twp., New Jersey, USA [Click for map]

(coordinates hidden from public) 26m

Who: I. G. Safonov (IGSafonov)

Specimen available

Notes:
[Notes updated/edited on 30-Oct-20 — IGS]
A NJMA foray collection by Lynn and Paul Hugerich.
Three fruitbodies of similar size and appearance growing in the vicinity of each other in a boggy area. Naturally, birch had to be nearby. This general area produced obs 294653 and obs 294666 two years prior, which are not the same as MO390060.
I now have sequencing data (ITS) for the present collection, which is to be discussed in detail later. In short, it turns out to be close to the European L. holopus. Den Bakker & Noordeloos (2007) discuss phylogeographic patterns in ITS and Gadph seen in this holarctic species.
The gory details of macro-morphology are evident from the images. The exposed flesh slowly stained salmon-red in the upper stipe; the cap context didn’t stain at all. The were some yellow and greenish-blue spots in the flesh at the very bottom of the stipe (see pix). The staining profile is in line with those of the two collections from 2017. The intensity of color and onset of staining are apparently directly related to the moisture content in the flesh.
The various dimensions of each fruitbody were measured separately, but they are very similar and I report them here as a range:
Cap diameter (=cap footprint): 3.5-4.0 cm
Cap diameter (measured across the cap surface): 5.2-5.4 cm
Cap thickness above stipe: 0.8-1.0 cm
Maximum hymenophore depth: 1.0-1.2 cm
Stipe length: 9.5-10.5 cm
Stipe width at cap juncture: 1.1 cm
Minimal stipe width (just below the pores): 0.75-0.90 cm
Stipe width in middle: 1.1-1.3 cm
Stipe width in bulb (=max width): 1.6-1.9 cm
None of the fruitbodies dropped spores.

Species Lists

Images

Photographed at ID session, 1-1.5 hr post collecting; natural light, no flash
Photographed at ID session, 1-1.5 hr post collecting; natural light, no flash
Photographed at ID session, 1-1.5 hr post collecting; natural light, no flash
Photographed at ID session, 1-1.5 hr post collecting; natural light, no flash
Photographed at ID session, 1-1.5 hr post collecting; natural light, no flash
Photographed ~6 hrs post collecting; natural light, no flash
Photographed ~6 hrs post collecting; natural light, no flash.
Context at ~20 sec following sectioning.
Photographed ~6 hrs post collecting; natural light, no flash.
Context at ~10 min following sectioning.

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Comments

Add Comment
Re holopus ID
By: I. G. Safonov (IGSafonov)
2019-10-27 23:07:44 MDT (-0600)

Surely looks like some of the pix in https://www.mushroomexpert.com/leccinum_holopus.html and the habitat (wet,
acidic soil) is a good match, too.
This collection will be submitted for sequencing. However,if it turns out to be conspecific with obs 294666, then it cannot be holopus.