Observation 44659: Lycoperdon pyriforme Schaeff.

When: 2010-04-23

Collection location: Crowsnest Pass, Alberta, Canada [Click for map]

Who: Johannes Harnisch (Johann Harnisch)

No specimen available


Copyright © 2010 Johannes Harnisch
Copyright © 2010 Johannes Harnisch
Copyright © 2010 Johannes Harnisch
Copyright © 2010 Johannes Harnisch
Copyright © 2010 Johannes Harnisch
Copyright © 2010 Johannes Harnisch
Copyright © 2010 Johannes Harnisch

Proposed Names

50% (1)
Recognized by sight
Based on microscopic features: smooth yellow olive spherical spores

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= Observer’s choice
= Current consensus


Add Comment
Spore projections
By: EP (Trailjunky)
2015-10-07 04:22:08 PDT (-0700)

I think some of the spores may have germinated.

I see
By: Johannes Harnisch (Johann Harnisch)
2010-04-25 13:13:55 PDT (-0700)

Thanks for the help…I am looking for cotton blue to buy then, haven’t had much success yet though.

I’m not a chemist
By: Irene Andersson (irenea)
2010-04-25 13:06:19 PDT (-0700)

and don’t know how methylene blue can be used.
It’s not the same as cotton blue (in lactic acid or lactophenol), which stains cyanophilous spores (walls and their ornaments) on certain kinds of mushrooms. I use it on spores from puffballs and coral fungi.

A better word for a preparate – is it a mount? I put spores or a piece of the mushroom on the slide. To that I add cotton blue and the cover slip. The reaction can take a while, but to get a better and faster result, I’m using a cigarrette lighter under the slide to heat it (being careful not to heat it too hard so the water evaporates).

Thin and dense spines can be seen in the full size pictures here, particularly on the smaller puffball. On a Lycoperdon perlatum of that age, we should have seen naked patches after fallen spines, surrounded by small warts in a netlike pattern.

these are last years though?
By: Johannes Harnisch (Johann Harnisch)
2010-04-25 11:27:26 PDT (-0700)

how can you see dense spines? or do you mean the spores?…
I was wondering what you mean by preparate?
do you suppose methylene blue would work or is of any use for fungi stains?
Thanks for your help I am really getting in to this microscopic stuff now! it is fun…

Thank you I will have to try that, I am sure it will be of great help….
By: Johannes Harnisch (Johann Harnisch)
2010-04-25 08:49:30 PDT (-0700)

the species in the picture are not the exact ones that the spores are of but a nearby specimen instead that looked the same, may be I should put a pic of that one on too…

Hard to tell
By: Irene Andersson (irenea)
2010-04-24 01:11:21 PDT (-0700)

how warty the spores are. A trick to get a better picture of the ornaments, is to add cotton blue to the preparate and heat it from below.

The dense and tiny spines on the puffball makes it look like something else than perlatum, but I don’t know what.

do the
By: Johannes Harnisch (Johann Harnisch)
2010-04-23 18:22:51 PDT (-0700)

spores look like they are minutely warty? there appears to be an stem like projection on some spores too…

Created: 2010-04-23 18:17:24 PDT (-0700)
Last modified: 2015-10-07 04:20:47 PDT (-0700)
Viewed: 91 times, last viewed: 2019-02-03 00:49:57 PST (-0800)
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